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nr recombinant human neuregulin 3 r d systems  (R&D Systems)


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    Structured Review

    R&D Systems nr recombinant human neuregulin 3 r d systems
    Nr Recombinant Human Neuregulin 3 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+nrg1/Recombinant+Human+NRG1%2FHRG1+Protein%2C+CF/10__1016_slash_j__cpblue__2026__100015-232-157-161
    Average 93 stars, based on 40 article reviews
    nr recombinant human neuregulin 3 r d systems - by Bioz Stars, 2026-09
    93/100 stars

    Images

    Related Articles

    Control:

    Article Title: A glycolytic shift in Schwann cells supports injured axons
    Article Snippet: MACS-purified mouse SCs from approximately 20 dissociated sciatic nerve preparations were seeded in PLL/laminin-coated 35 mm dishes and cultured for ~20h in defined medium (10% Normal horse Serum (Gibco, #26050-088) in DMEM/F12, 350μg/mL BSA (Sigma, A4161), 1μM insulin (Sigma, I6634)) in preparation for ErbB2 receptor activation with recombinant Nrg1 according to a previously published method 71 . .. SCs were subsequently control-treated or treated with 200 ng/ml recombinant Nrg1 (R&D Systems, 396-HB-050) for 24h, collected in RIPA buffer containing phosphatase and protease inhibitors, and then processed for protein analysis and western blotting using standard procedures. ..

    Recombinant:

    Article Title: A glycolytic shift in Schwann cells supports injured axons
    Article Snippet: MACS-purified mouse SCs from approximately 20 dissociated sciatic nerve preparations were seeded in PLL/laminin-coated 35 mm dishes and cultured for ~20h in defined medium (10% Normal horse Serum (Gibco, #26050-088) in DMEM/F12, 350μg/mL BSA (Sigma, A4161), 1μM insulin (Sigma, I6634)) in preparation for ErbB2 receptor activation with recombinant Nrg1 according to a previously published method 71 . .. SCs were subsequently control-treated or treated with 200 ng/ml recombinant Nrg1 (R&D Systems, 396-HB-050) for 24h, collected in RIPA buffer containing phosphatase and protease inhibitors, and then processed for protein analysis and western blotting using standard procedures. ..

    Article Title: Populations of Radial Glial Cells Respond Differently to Reelin and Neuregulin1 in a Ferret Model of Cortical Dysplasia
    Article Snippet: In some experiments, the medium was supplemented with recombinant mouse reelin (1 nM, US Biological, Swampscott, MA) corresponding to the central fragment. .. We also used recombinant NRG1 (1 nM, R&D systems, Minneapolis, MN) obtained from the Human DNA sequence encoding the EGF domain of NRG1 β1. .. To further understand the effects mediated by reelin or NRG1, the culture medium was complemented with pathway inhibitors such as: LY294002 (inhibitor of PI3K; 50 μM, Calbiochem, San Diego, CA), PP2 (a Src kinases inhibitor; 10 μM, Calbiochem), TDZD-8 (a GSK-3β Inhibitor I, 56 μM, Calbiochem), or SP600125 (a JNK Inhibitor II, 10 μM, Calbiochem).

    Article Title: Src and ADAM-17–Mediated Shedding of Transforming Growth Factor-α Is a Mechanism of Acute Resistance to TRAIL
    Article Snippet: Recombinant TGFa was purchased from Calbiochem and reconstituted to a concentration of 50 Ag/mL in 10 mmol/L acetic acid containing 0.1% BSA and stored at 70jC. .. Recombinant NRG1 was obtained from R&D Systems and reconstituted to a concentration of 25 Ag/mL in BPS containing 0.1% BSA and stored at 20jC. ..

    Article Title: Acute doxorubicin cardiotoxicity is associated with miR-146a-induced inhibition of the neuregulin-ErbB pathway
    Article Snippet: Anti-bcl-2 antibody (83-8B) was obtained from MBL (Nagoya, Japan). .. Recombinant NRG1- 1 (396-HB) was obtained from R&D Systems (MN, USA). .. Tetramethylrhodamine, ethylester, perchlorate (TMRE) was obtained from Molecular Probes (OR, USA).

    Article Title: Dataset on the effect of knockout of KLK8 in social memory
    Article Snippet: .. 2 μl of 0.5 nM recombinant NRG1 177-246 [human NRG1-β1/HRG1-β1 EGF domain CF, R&D Systems, Minnesota, U.S.A.; human NRG1 177-246 shares 98.6% (100%) sequence identity (similarity) with the mouse NRG1 177-246 ] was injected into the cerebral ventricle for 10 min. After injection, the mice were placed in the three-chamber apparatus for 20 min for habituation, and the behavioral test was subsequently performed. ..

    Article Title: A genetic compensatory mechanism regulated by Jun and Mef2d modulates the expression of distinct class IIa Hdacs to ensure peripheral nerve myelination and repair
    Article Snippet: .. After 72 hr, the medium was removed and cell cultures expanded in DMEM supplemented with 3% fetal bovine serum, 5 μM forskolin, and 10 ng/ml recombinant NRG1 (R&D Systems). .. Where indicated, cells were incubated in SATO medium (composed of a 1:1 mixture of DMEM and Ham’s F12 medium [Gibco] supplemented with ITS [1:100; Gibco]), 0.1 mM putrescine, and 20 nM of progesterone (Bottenstein and Sato, 1979).

    Western Blot:

    Article Title: A glycolytic shift in Schwann cells supports injured axons
    Article Snippet: MACS-purified mouse SCs from approximately 20 dissociated sciatic nerve preparations were seeded in PLL/laminin-coated 35 mm dishes and cultured for ~20h in defined medium (10% Normal horse Serum (Gibco, #26050-088) in DMEM/F12, 350μg/mL BSA (Sigma, A4161), 1μM insulin (Sigma, I6634)) in preparation for ErbB2 receptor activation with recombinant Nrg1 according to a previously published method 71 . .. SCs were subsequently control-treated or treated with 200 ng/ml recombinant Nrg1 (R&D Systems, 396-HB-050) for 24h, collected in RIPA buffer containing phosphatase and protease inhibitors, and then processed for protein analysis and western blotting using standard procedures. ..

    Sequencing:

    Article Title: Populations of Radial Glial Cells Respond Differently to Reelin and Neuregulin1 in a Ferret Model of Cortical Dysplasia
    Article Snippet: In some experiments, the medium was supplemented with recombinant mouse reelin (1 nM, US Biological, Swampscott, MA) corresponding to the central fragment. .. We also used recombinant NRG1 (1 nM, R&D systems, Minneapolis, MN) obtained from the Human DNA sequence encoding the EGF domain of NRG1 β1. .. To further understand the effects mediated by reelin or NRG1, the culture medium was complemented with pathway inhibitors such as: LY294002 (inhibitor of PI3K; 50 μM, Calbiochem, San Diego, CA), PP2 (a Src kinases inhibitor; 10 μM, Calbiochem), TDZD-8 (a GSK-3β Inhibitor I, 56 μM, Calbiochem), or SP600125 (a JNK Inhibitor II, 10 μM, Calbiochem).

    Article Title: Dataset on the effect of knockout of KLK8 in social memory
    Article Snippet: .. 2 μl of 0.5 nM recombinant NRG1 177-246 [human NRG1-β1/HRG1-β1 EGF domain CF, R&D Systems, Minnesota, U.S.A.; human NRG1 177-246 shares 98.6% (100%) sequence identity (similarity) with the mouse NRG1 177-246 ] was injected into the cerebral ventricle for 10 min. After injection, the mice were placed in the three-chamber apparatus for 20 min for habituation, and the behavioral test was subsequently performed. ..

    Concentration Assay:

    Article Title: Src and ADAM-17–Mediated Shedding of Transforming Growth Factor-α Is a Mechanism of Acute Resistance to TRAIL
    Article Snippet: Recombinant TGFa was purchased from Calbiochem and reconstituted to a concentration of 50 Ag/mL in 10 mmol/L acetic acid containing 0.1% BSA and stored at 70jC. .. Recombinant NRG1 was obtained from R&D Systems and reconstituted to a concentration of 25 Ag/mL in BPS containing 0.1% BSA and stored at 20jC. ..

    Injection:

    Article Title: Dataset on the effect of knockout of KLK8 in social memory
    Article Snippet: .. 2 μl of 0.5 nM recombinant NRG1 177-246 [human NRG1-β1/HRG1-β1 EGF domain CF, R&D Systems, Minnesota, U.S.A.; human NRG1 177-246 shares 98.6% (100%) sequence identity (similarity) with the mouse NRG1 177-246 ] was injected into the cerebral ventricle for 10 min. After injection, the mice were placed in the three-chamber apparatus for 20 min for habituation, and the behavioral test was subsequently performed. ..



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    AHR-activated macrophages impair trophoblast function. ( A ) Heatmap showing expression levels of CGAS , NLRP3 , and TLR9 in various cell types determined by scRNA-seq. ( B ) Bar plots displaying significantly enriched GO terms in <t>EVTs</t> from URPL pregnancies. ( C ) Schematic diagram illustrating the co-culture system. ( D ) Representative images western blot images of TLR9, NLRP3, and cGAS protein expression in primary <t>EVTs</t> <t>co-cultured</t> with KYN-pretreated dMφs or control dMφs (n = 3 per group). ( E ) Representative immunofluorescence images showing co-localization of HLA-G and cGAS in hTSCs-EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 5 per group). ( F ) Flow cytometry analysis of apoptosis levels in primary EVTs following co-culture with dMφs (n = 3 per group). ( G ) Scratch wound healing assay evaluating the migration capacity of HTR-8/SVneo cells (n = 3 per group). ( H ) Transwell invasion assay measuring the invasive ability of HTR-8/SVneo cells (n = 3 per group). ( I ) Western blot analysis of proteins associated with NF-κB signaling (p-NF-κB p65, NF-κB p65, p-IκBα, IκBα), apoptosis (BCL2, cleaved Caspase-3), and extracellular matrix remodeling (MMP9, MMP2) in HTR-8/SVneo cells. Data are presented as mean ± SD. Statistical significance was determined using the Student's t-test for two-group comparisons and one-way ANOVA for multiple comparisons; ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.
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    Image Search Results


    AHR-activated macrophages impair trophoblast function. ( A ) Heatmap showing expression levels of CGAS , NLRP3 , and TLR9 in various cell types determined by scRNA-seq. ( B ) Bar plots displaying significantly enriched GO terms in EVTs from URPL pregnancies. ( C ) Schematic diagram illustrating the co-culture system. ( D ) Representative images western blot images of TLR9, NLRP3, and cGAS protein expression in primary EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 3 per group). ( E ) Representative immunofluorescence images showing co-localization of HLA-G and cGAS in hTSCs-EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 5 per group). ( F ) Flow cytometry analysis of apoptosis levels in primary EVTs following co-culture with dMφs (n = 3 per group). ( G ) Scratch wound healing assay evaluating the migration capacity of HTR-8/SVneo cells (n = 3 per group). ( H ) Transwell invasion assay measuring the invasive ability of HTR-8/SVneo cells (n = 3 per group). ( I ) Western blot analysis of proteins associated with NF-κB signaling (p-NF-κB p65, NF-κB p65, p-IκBα, IκBα), apoptosis (BCL2, cleaved Caspase-3), and extracellular matrix remodeling (MMP9, MMP2) in HTR-8/SVneo cells. Data are presented as mean ± SD. Statistical significance was determined using the Student's t-test for two-group comparisons and one-way ANOVA for multiple comparisons; ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: International Journal of Biological Sciences

    Article Title: Excessive Kynurenine Metabolism Impairs Lysosomal acidification and Triggers mtDNA Release via the AHR/CISH/ATP6V1A Axis in Decidual Macrophages Associated with Unexplained Recurrent Pregnancy Loss

    doi: 10.7150/ijbs.121947

    Figure Lengend Snippet: AHR-activated macrophages impair trophoblast function. ( A ) Heatmap showing expression levels of CGAS , NLRP3 , and TLR9 in various cell types determined by scRNA-seq. ( B ) Bar plots displaying significantly enriched GO terms in EVTs from URPL pregnancies. ( C ) Schematic diagram illustrating the co-culture system. ( D ) Representative images western blot images of TLR9, NLRP3, and cGAS protein expression in primary EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 3 per group). ( E ) Representative immunofluorescence images showing co-localization of HLA-G and cGAS in hTSCs-EVTs co-cultured with KYN-pretreated dMφs or control dMφs (n = 5 per group). ( F ) Flow cytometry analysis of apoptosis levels in primary EVTs following co-culture with dMφs (n = 3 per group). ( G ) Scratch wound healing assay evaluating the migration capacity of HTR-8/SVneo cells (n = 3 per group). ( H ) Transwell invasion assay measuring the invasive ability of HTR-8/SVneo cells (n = 3 per group). ( I ) Western blot analysis of proteins associated with NF-κB signaling (p-NF-κB p65, NF-κB p65, p-IκBα, IκBα), apoptosis (BCL2, cleaved Caspase-3), and extracellular matrix remodeling (MMP9, MMP2) in HTR-8/SVneo cells. Data are presented as mean ± SD. Statistical significance was determined using the Student's t-test for two-group comparisons and one-way ANOVA for multiple comparisons; ns: not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: To induce hTSCs-derived EVTs (hTSCs-EVTs), hTSCs were seeded onto 6-well plates pre-coated with 1 μg/ml collagen IV and cultured in 2 mL of EVTs medium [DMEM/F12 supplemented with 0.1 mM 2-mercaptoethanol, 0.5% PS, 0.3% BSA, 1% ITS-X, 100 ng/ml NRG1 (CST, Cat#26941), 7.5 μM A83-01, 2.5 μM Y27632, and 4% KnockOut Serum Replacement (KSR, ThermoFisher, Cat#10828010)].

    Techniques: Expressing, Co-Culture Assay, Western Blot, Cell Culture, Control, Immunofluorescence, Flow Cytometry, Wound Healing Assay, Migration, Transwell Invasion Assay